Poster #RP203
A Phylogenetic Study of uvrB Gene.
Uaska Bezerra*, Lucymara Agnez-lima*
*UFRN, NATAL, BRAZIL
The phylogenomic aims the evolutionary analysis of genome sequences across comparative analysis of conserved sequences and search of lateral gene transfer. DNA repair genes are important for the genetic stability and have a high conservation degree. Variations at nucleotide excision repair (NER) genes may indicate a horizontal gene transfer. The objective of this work was to perform a phylogenetic analysis of uvrB gene, which is a subunit of ABC excinuclease complex. The protein sequences were obtained by comparison of the uvrB sequence from Chromobacterium violaceum with sequences from the GenBank through Blast-p software using 10-15 cutoff. A Databank was created for realize phylogenetic analysis using computer algorithms implemented in PHYLIP package and PAUP program. Trees of uvrB and rDNA sequences were generated through parsimony, distance and neighbor joining methods. At uvrB tree, was observed that three species of Epsilonproteobarcterias class, three species of Mollicutes class and two representatives of archaeal bacteria (Methanosarcina barkeri and Methanococcus maripaludis) form a monophyletic group what could be explained by occurrence of horizontal transfer gene. Its possible infers that the common ancestor of this gene would be from an archaeal bacteria, therefore the nucleotide excision repair genes in M.barkeri and M.maripaludis are organized in operon.