Poster #RP204
Phage integrases repertoire in Xylella fastidiosa : A comparative study using a BBH approach
Alessandro de Mello Varani*, Marie-Anne Van Sluys**, Rangel Celso Souza***
*GaTE Laboratory - Institute of Bioscieces - University of São Paulo (IB-USP), São Paulo, Brazil; **GaTE Laboratory - Institute of Bioscieces - University of São Paulo (IB-USP) , São Paulo, Brazil; ***Laboratório Nacional de Computação Científica (LNCC/RJ), Petrópolis, Brazil
Xylella fastidiosa is a gamma-proteobacterium that causes plant diseases. Genomic differences among distinct strains are mainly associated to bacteriophages chromosomal rearrangements, insertions or deletions. In order to occur the integration of any bacteriophage into the host genome, activity of phage-specific integrase is essential. To understand the evolutionary history of Xylella prophages a tool based on SABIÁ Software was developed to compare the Xylella prophage proteome agaisnt 142 complete genome sequences of active phages deposited at GenBank. A BBH study was performed to identify relations among the phage-integrases. Eleven clusters were detected when using 70% of alignment coverage. Among these, Xylella phage-integrases fall in two clusters containing a total of 68 proteins. The first cluster contain eight phage-integrases, being five Xylella phage-integrases with close relation to integrases from temperate Enterobacteria lambda phages of Caudovirales. The second cluster contains one Xylella phage-integrase related to a genomic island and 59 phage-integrases. The main differences between theses clusters are related to the fact that most of phages inclued in the second cluster have a major capacity to enter the cycle lytic convertion. Nonetheless, full lenght proteome comparisons support the mosaic nature of Xylella prophage regions.