Poster #RP225
Proteomic Profiling of Methicillin Sensitive (MSSA) and Methicillin Resistant (MRSA) Staphylococcus Aureus Strains by LC/MS and LC/MS/MS
Ronan OMalley*, Therese McKenna*, H.N. Shah**
*Waters Corp., Manchester, England; **Health Protection Agency, London, England
A label free, exact mass, mass spectrometry-based approach was used to analyze proteomic profiles from MSSA and MRSA and could be used to identify potential protein markers for strain differentiation. The detection of a lipoprotein biomarker for MRSA and MSSA strains from a total tryptic digest, to our knowledge represents the first such finding and provides a novel approach for further studies. No prior preparative steps such as 2-D gel electrophoresis nor fractionation methods were undertaken. Future studies, involving a larger number of strains will establish the confidence limits of this biomarker among drug-resistant strains of S.aureus and its potential use for the clinical diagnostic laboratory.