Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.
Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
CF568 Arachis hypogaes Lectin PNA (Peanut) |
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29061 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF594 Arachis hypogaes Lectin PNA (Peanut) |
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29062 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF488A Arachis hypogaes Lectin PNA (Peanut) |
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29060 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF640R Arachis hypogaes Lectin PNA (Peanut) |
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29063 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
Arachin Ahy-3 Antibody, HRP conjugated |
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1-CSB-PA737391LB01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is HRP conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Polyclonal Antibody, HRP Conjugated |
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A56271 | EpiGentek |
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QPCR Kit DNA Arachis hyp |
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MOL6482 | Scientific Laboratory Supplies | EACH | EUR 1118.9 |
QPCR Kit DNA Arachis hyp Mmix |
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MOL6484 | Scientific Laboratory Supplies | EACH | EUR 3461.15 |
BSA Conjugated Arachidonic Acid (AA) |
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4-CPB098Ge11 | Cloud-Clone |
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Description: Recombinant Pan-species Arachidonic Acid expressed in: Protein conjugation |
OVA Conjugated Arachidonic Acid (AA) |
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4-CPB098Ge21 | Cloud-Clone |
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Description: Recombinant Pan-species Arachidonic Acid expressed in: chemical synthesis |
Arachin Ahy-3 Antibody, FITC conjugated |
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1-CSB-PA737391LC01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is FITC conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Antibody, Biotin conjugated |
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1-CSB-PA737391LD01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Polyclonal Antibody, FITC Conjugated |
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A56272 | EpiGentek |
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Arachin Ahy-3 Polyclonal Antibody, Biotin Conjugated |
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A56273 | EpiGentek |
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HYPM Antibody, HRP conjugated |
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1-CSB-PA006268LB01HU | Cusabio |
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Description: A polyclonal antibody against HYPM. Recognizes HYPM from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ARAF Antibody, HRP conjugated |
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1-CSB-PA01679B0Rb | Cusabio |
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Description: A polyclonal antibody against ARAF. Recognizes ARAF from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ARAP3 Antibody, HRP conjugated |
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1-CSB-PA819904LB01HU | Cusabio |
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Description: A polyclonal antibody against ARAP3. Recognizes ARAP3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ARAP1 Antibody, HRP conjugated |
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1-CSB-PA856998LB01HU | Cusabio |
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Description: A polyclonal antibody against ARAP1. Recognizes ARAP1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
HYPM Polyclonal Antibody, HRP Conjugated |
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A62359 | EpiGentek |
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HRP Conjugated Avidin |
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BA1081-0.5 | BosterBio | 0.5ml | EUR 175.2 |
HRP Conjugated Avidin |
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BA1081-1 | BosterBio | 1ml | EUR 279.6 |
HRP Conjugated Streptavidin |
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BA1088-0.5 | BosterBio | 0.5ml | EUR 175.2 |
HRP Conjugated Streptavidin |
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BA1088-1 | BosterBio | 1ml | EUR 279.6 |
Streptavidin HRP Conjugated |
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LS1012 | SAB | 1.0 ml | EUR 142.8 |
Streptavidin, HRP conjugated |
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S0900-001 | GenDepot | 1mg | EUR 360 |
HRP Conjugated Protein A |
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BA1080-0.5 | BosterBio | 0.5ml | EUR 224.4 |
HRP Conjugated Protein A |
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BA1080-1 | BosterBio | 1ml | EUR 400.8 |
CS Antibody, HRP conjugated |
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1-CSB-PA006031LB01HU | Cusabio |
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Description: A polyclonal antibody against CS. Recognizes CS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
TG Antibody, HRP conjugated |
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1-CSB-PA12325B0Rb | Cusabio |
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Description: A polyclonal antibody against TG. Recognizes TG from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |